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mixed-effects cosinor analysis  (SAS institute)


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    SAS institute mixed-effects cosinor analysis
    (A) Healthy human subjects were studied in-laboratory and subjected to 3 days of a simulated day shift schedule ( B , control condition) or a simulated night shift condition ( C , experimental condition). This was followed by a 24-hour constant routine protocol during which blood was drawn at 3-hour intervals (ZT2, ZT5, etc.) Blood samples collected at ZT2 and ZT14 (8 AM and 8 PM, respectively, in the day shift condition, or 8 PM and 8 AM in the night shift condition) were immediately treated with 10 μM cisplatin. Blood samples were incubated and fractions were collected between 2 and 24 hours later to isolate PBMCs. Genomic DNA was purified and probed for cisplatin-DNA adduct levels with an α-Pt-(GpG) antibody using a slot-blot assay. (D) mRNA was isolated from the blood samples and gene expression for XPA, the rate-limiting factor in NER, was analyzed using the NanoString multiplex assay. (E) DNA-protein interaction between the Bmal1 and XPA is shown in the first 3,000 base pair promoter region of human melanoma SKMEL-27 cells using a ChIP assay. PER2 is a circadian clock positive control. Input and IgG are experimental positive and negative controls, respectively. (F) Quantitation of Bmal1 binding to promoter regions of PER2 and XPA from ChIP assay, indicating regions of significance after IgG binding subtraction. Statistical analysis was done using two-way ANOVA with n=3 subjects per group (B-C) and <t>cosinor</t> analysis with n=7 subjects per group (D), and t test with n=3 replicates for E-F. * =p<0.05 for circadian rhythmicity or ChIP binding. Error bars = S.E.M.
    Mixed Effects Cosinor Analysis, supplied by SAS institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mixed-effects+cosinor+analysis/mixed+effects+cosinor+analysis/pmc05865687-249-6-9
    Average 90 stars, based on 1 article reviews
    mixed-effects cosinor analysis - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "The circadian clock regulates cisplatin-induced toxicity and tumor regression in melanoma mouse and human models"

    Article Title: The circadian clock regulates cisplatin-induced toxicity and tumor regression in melanoma mouse and human models

    Journal: Oncotarget

    doi: 10.18632/oncotarget.24539

    (A) Healthy human subjects were studied in-laboratory and subjected to 3 days of a simulated day shift schedule ( B , control condition) or a simulated night shift condition ( C , experimental condition). This was followed by a 24-hour constant routine protocol during which blood was drawn at 3-hour intervals (ZT2, ZT5, etc.) Blood samples collected at ZT2 and ZT14 (8 AM and 8 PM, respectively, in the day shift condition, or 8 PM and 8 AM in the night shift condition) were immediately treated with 10 μM cisplatin. Blood samples were incubated and fractions were collected between 2 and 24 hours later to isolate PBMCs. Genomic DNA was purified and probed for cisplatin-DNA adduct levels with an α-Pt-(GpG) antibody using a slot-blot assay. (D) mRNA was isolated from the blood samples and gene expression for XPA, the rate-limiting factor in NER, was analyzed using the NanoString multiplex assay. (E) DNA-protein interaction between the Bmal1 and XPA is shown in the first 3,000 base pair promoter region of human melanoma SKMEL-27 cells using a ChIP assay. PER2 is a circadian clock positive control. Input and IgG are experimental positive and negative controls, respectively. (F) Quantitation of Bmal1 binding to promoter regions of PER2 and XPA from ChIP assay, indicating regions of significance after IgG binding subtraction. Statistical analysis was done using two-way ANOVA with n=3 subjects per group (B-C) and cosinor analysis with n=7 subjects per group (D), and t test with n=3 replicates for E-F. * =p<0.05 for circadian rhythmicity or ChIP binding. Error bars = S.E.M.
    Figure Legend Snippet: (A) Healthy human subjects were studied in-laboratory and subjected to 3 days of a simulated day shift schedule ( B , control condition) or a simulated night shift condition ( C , experimental condition). This was followed by a 24-hour constant routine protocol during which blood was drawn at 3-hour intervals (ZT2, ZT5, etc.) Blood samples collected at ZT2 and ZT14 (8 AM and 8 PM, respectively, in the day shift condition, or 8 PM and 8 AM in the night shift condition) were immediately treated with 10 μM cisplatin. Blood samples were incubated and fractions were collected between 2 and 24 hours later to isolate PBMCs. Genomic DNA was purified and probed for cisplatin-DNA adduct levels with an α-Pt-(GpG) antibody using a slot-blot assay. (D) mRNA was isolated from the blood samples and gene expression for XPA, the rate-limiting factor in NER, was analyzed using the NanoString multiplex assay. (E) DNA-protein interaction between the Bmal1 and XPA is shown in the first 3,000 base pair promoter region of human melanoma SKMEL-27 cells using a ChIP assay. PER2 is a circadian clock positive control. Input and IgG are experimental positive and negative controls, respectively. (F) Quantitation of Bmal1 binding to promoter regions of PER2 and XPA from ChIP assay, indicating regions of significance after IgG binding subtraction. Statistical analysis was done using two-way ANOVA with n=3 subjects per group (B-C) and cosinor analysis with n=7 subjects per group (D), and t test with n=3 replicates for E-F. * =p<0.05 for circadian rhythmicity or ChIP binding. Error bars = S.E.M.

    Techniques Used: Control, Incubation, Purification, Slot Blot Assay, Isolation, Gene Expression, Multiplex Assay, Positive Control, Quantitation Assay, Binding Assay

    Related Articles

    Control:

    Article Title: The circadian clock regulates cisplatin-induced toxicity and tumor regression in melanoma mouse and human models
    Article Snippet: Oscillations of genes were analyzed using mixed-effects cosinor analysis (SAS software) [ ].

    Incubation:

    Article Title: The circadian clock regulates cisplatin-induced toxicity and tumor regression in melanoma mouse and human models
    Article Snippet: Oscillations of genes were analyzed using mixed-effects cosinor analysis (SAS software) [ ].

    Purification:

    Article Title: The circadian clock regulates cisplatin-induced toxicity and tumor regression in melanoma mouse and human models
    Article Snippet: Oscillations of genes were analyzed using mixed-effects cosinor analysis (SAS software) [ ].

    Slot Blot Assay:

    Article Title: The circadian clock regulates cisplatin-induced toxicity and tumor regression in melanoma mouse and human models
    Article Snippet: Oscillations of genes were analyzed using mixed-effects cosinor analysis (SAS software) [ ].

    Isolation:

    Article Title: The circadian clock regulates cisplatin-induced toxicity and tumor regression in melanoma mouse and human models
    Article Snippet: Oscillations of genes were analyzed using mixed-effects cosinor analysis (SAS software) [ ].

    Gene Expression:

    Article Title: The circadian clock regulates cisplatin-induced toxicity and tumor regression in melanoma mouse and human models
    Article Snippet: Oscillations of genes were analyzed using mixed-effects cosinor analysis (SAS software) [ ].

    Multiplex Assay:

    Article Title: The circadian clock regulates cisplatin-induced toxicity and tumor regression in melanoma mouse and human models
    Article Snippet: Oscillations of genes were analyzed using mixed-effects cosinor analysis (SAS software) [ ].

    Positive Control:

    Article Title: The circadian clock regulates cisplatin-induced toxicity and tumor regression in melanoma mouse and human models
    Article Snippet: Oscillations of genes were analyzed using mixed-effects cosinor analysis (SAS software) [ ].

    Quantitation Assay:

    Article Title: The circadian clock regulates cisplatin-induced toxicity and tumor regression in melanoma mouse and human models
    Article Snippet: Oscillations of genes were analyzed using mixed-effects cosinor analysis (SAS software) [ ].

    Binding Assay:

    Article Title: The circadian clock regulates cisplatin-induced toxicity and tumor regression in melanoma mouse and human models
    Article Snippet: Oscillations of genes were analyzed using mixed-effects cosinor analysis (SAS software) [ ].



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    mixed-effects cosinor analysis  (SAS institute)
    90
    SAS institute mixed-effects cosinor analysis
    (A) Healthy human subjects were studied in-laboratory and subjected to 3 days of a simulated day shift schedule ( B , control condition) or a simulated night shift condition ( C , experimental condition). This was followed by a 24-hour constant routine protocol during which blood was drawn at 3-hour intervals (ZT2, ZT5, etc.) Blood samples collected at ZT2 and ZT14 (8 AM and 8 PM, respectively, in the day shift condition, or 8 PM and 8 AM in the night shift condition) were immediately treated with 10 μM cisplatin. Blood samples were incubated and fractions were collected between 2 and 24 hours later to isolate PBMCs. Genomic DNA was purified and probed for cisplatin-DNA adduct levels with an α-Pt-(GpG) antibody using a slot-blot assay. (D) mRNA was isolated from the blood samples and gene expression for XPA, the rate-limiting factor in NER, was analyzed using the NanoString multiplex assay. (E) DNA-protein interaction between the Bmal1 and XPA is shown in the first 3,000 base pair promoter region of human melanoma SKMEL-27 cells using a ChIP assay. PER2 is a circadian clock positive control. Input and IgG are experimental positive and negative controls, respectively. (F) Quantitation of Bmal1 binding to promoter regions of PER2 and XPA from ChIP assay, indicating regions of significance after IgG binding subtraction. Statistical analysis was done using two-way ANOVA with n=3 subjects per group (B-C) and <t>cosinor</t> analysis with n=7 subjects per group (D), and t test with n=3 replicates for E-F. * =p<0.05 for circadian rhythmicity or ChIP binding. Error bars = S.E.M.
    Mixed Effects Cosinor Analysis, supplied by SAS institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mixed-effects+cosinor+analysis/mixed+effects+cosinor+analysis/pmc05865687-249-6-9
    Average 90 stars, based on 1 article reviews
    mixed-effects cosinor analysis - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Healthy human subjects were studied in-laboratory and subjected to 3 days of a simulated day shift schedule ( B , control condition) or a simulated night shift condition ( C , experimental condition). This was followed by a 24-hour constant routine protocol during which blood was drawn at 3-hour intervals (ZT2, ZT5, etc.) Blood samples collected at ZT2 and ZT14 (8 AM and 8 PM, respectively, in the day shift condition, or 8 PM and 8 AM in the night shift condition) were immediately treated with 10 μM cisplatin. Blood samples were incubated and fractions were collected between 2 and 24 hours later to isolate PBMCs. Genomic DNA was purified and probed for cisplatin-DNA adduct levels with an α-Pt-(GpG) antibody using a slot-blot assay. (D) mRNA was isolated from the blood samples and gene expression for XPA, the rate-limiting factor in NER, was analyzed using the NanoString multiplex assay. (E) DNA-protein interaction between the Bmal1 and XPA is shown in the first 3,000 base pair promoter region of human melanoma SKMEL-27 cells using a ChIP assay. PER2 is a circadian clock positive control. Input and IgG are experimental positive and negative controls, respectively. (F) Quantitation of Bmal1 binding to promoter regions of PER2 and XPA from ChIP assay, indicating regions of significance after IgG binding subtraction. Statistical analysis was done using two-way ANOVA with n=3 subjects per group (B-C) and cosinor analysis with n=7 subjects per group (D), and t test with n=3 replicates for E-F. * =p<0.05 for circadian rhythmicity or ChIP binding. Error bars = S.E.M.

    Journal: Oncotarget

    Article Title: The circadian clock regulates cisplatin-induced toxicity and tumor regression in melanoma mouse and human models

    doi: 10.18632/oncotarget.24539

    Figure Lengend Snippet: (A) Healthy human subjects were studied in-laboratory and subjected to 3 days of a simulated day shift schedule ( B , control condition) or a simulated night shift condition ( C , experimental condition). This was followed by a 24-hour constant routine protocol during which blood was drawn at 3-hour intervals (ZT2, ZT5, etc.) Blood samples collected at ZT2 and ZT14 (8 AM and 8 PM, respectively, in the day shift condition, or 8 PM and 8 AM in the night shift condition) were immediately treated with 10 μM cisplatin. Blood samples were incubated and fractions were collected between 2 and 24 hours later to isolate PBMCs. Genomic DNA was purified and probed for cisplatin-DNA adduct levels with an α-Pt-(GpG) antibody using a slot-blot assay. (D) mRNA was isolated from the blood samples and gene expression for XPA, the rate-limiting factor in NER, was analyzed using the NanoString multiplex assay. (E) DNA-protein interaction between the Bmal1 and XPA is shown in the first 3,000 base pair promoter region of human melanoma SKMEL-27 cells using a ChIP assay. PER2 is a circadian clock positive control. Input and IgG are experimental positive and negative controls, respectively. (F) Quantitation of Bmal1 binding to promoter regions of PER2 and XPA from ChIP assay, indicating regions of significance after IgG binding subtraction. Statistical analysis was done using two-way ANOVA with n=3 subjects per group (B-C) and cosinor analysis with n=7 subjects per group (D), and t test with n=3 replicates for E-F. * =p<0.05 for circadian rhythmicity or ChIP binding. Error bars = S.E.M.

    Article Snippet: Oscillations of genes were analyzed using mixed-effects cosinor analysis (SAS software) [ ].

    Techniques: Control, Incubation, Purification, Slot Blot Assay, Isolation, Gene Expression, Multiplex Assay, Positive Control, Quantitation Assay, Binding Assay